Qiagen, 965752, QIAamp Media MDx Kit (12)

The QIAamp Media MDx Kit provides a fully automated procedure for purification of DNA from liquid media, such as cervical swab transport media. The fully automated process on the BioRobot MDx workstation, including bar code reading, load check, and complete process documentation, requires only 225 minutes for 96 samples, with no hands-on time.
Purified nucleic acids are free of proteins, nucleases, and other impurities. Highly reproducible yields enable accurate results in downstream assays (see figure "Highly reproducible yields"). The high-quality nucleic acids are ready to use in downstream assays, such as real-time PCR (see figure "Linear yields of high-quality nucleic acids").
The QIAamp Media MDx Kit uses well-established technology for purification of nucleic acids from liquid media. The kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, for fully automated, simultaneous processing of up to ninety-six 265 µl samples on the BioRobot MDx.
Liquid transport media can crosslink cells and form cellular aggregates that are difficult to lyse. Optimized buffers in the QIAamp Media MDx Kit efficiently lyse these samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane (see flowchart "Protocol"). Alcohol is added and lysates are loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities, including alcohols and phosphate, and pure, ready-to-use DNA is then eluted in a low-salt buffer. Patent-pending, computer-controlled vacuum technology ensures that volatile contaminants in liquid cytology media, such as alcohols, are efficiently removed.