Qiagen, WB129242, QIAcard FTA DMPK-B (100), 4 sample areas per card

QIAcard FTA DMPK formats offer a reliable and cost-effective sample collection technique for analyzing pharmacokinetics in clinical studies, global health surveillance programs and forensic applications. Simply apply a small sample of blood or other biofluid directly to the DMPK card and allow it to dry at room temperature – the sample is then stabilized on the card for later use. For downstream analyses, such as HPLC and MS/MS, a small disc can be punched out of the card to extract drugs and metabolites.
Analysis is primarily with HPLC or UHPLC followed by MS/MS detection, direct desorption MS techniques such as DESI. Methanol or aqueous/organic mixtures have been shown to work well for small molecules and aqueous buffers are recommended for proteins. Select a good solvent for the analyte while maintaining compatibility with the HPLC conditions to be used. The choice of DMPK card depends on many factors such as the analyte chemical structure, extraction solvent and analysis workflow. See "Workflow for Sample Analysis Procedure".