Stemcell, 02012, Human IL-10 ELISA Kit

The Human Interleukin 10 (IL-10) ELISA Kit is designed for the quantitative detection and measurement of human IL-10 in biological fluids such as serum, plasma, and cell culture supernatants. IL-10 is primarily produced by dendritic cells, macrophages, and CD4+ T cells. IL-10 is important for the function of regulatory T cells and it enhances B cell proliferation, immunoglobulin secretion, and MHC class II expression.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.