Stemcell, 04431, MethoCult™ H4431

MethoCult™ H4431 is a methylcelluose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. MethoCult™ H4431 supports the growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). This formulation contains agar leukocyte conditioned medium (agar-LCM) as a source of colony-stimulating factors, plus recombinant human erythropoietin (EPO). It is suitable for use as a control medium for detection of “EPO-independent” erythroid progenitor cells using MethoCult™ H4531 (Catalog #04531).

Browse our Frequently Asked Questions (FAQs) on performing the CFU assay and explore its utility as part of the cell therapy workflow.