Description
STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (Catalog #05010) includes a medium for differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells (human pluripotent stem cells [hPSCs]) into ventricular cardiomyocytes (cardiac troponin T-positive [cTnT+]), as well as a medium for maintenance of hPSC-derived cardiomyocytes. This serum-free kit can be used to generate ventricular cardiomyocytes derived from a clump culture of hPSCs maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), TeSR™-AOF (Catalog #100-0401), or TeSR™-E8™ (Catalog #05990). Greater than 80% of these cells will be cTnT+. An average of 1 x 10^6 cells can be harvested from a single well of a 12-well plate.
STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement; it can be used for long-term maintenance of hPSC-derived cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.
NOTE: This product was formerly named ‘STEMdiff™ Cardiomyocyte Differentiation Kit’; the product itself and manufacturing procedures have not changed, but the name has been updated to more accurately reflect the cell type generated.
