Stemcell, 05050, PneumaCult™-ALI-S Medium

PneumaCult™-ALI-S Medium (Catalog #05050) is a serum- and BPE-free medium for the culture of human small airway epithelial cells at the air-liquid interface (ALI). Small airway epithelial cells cultured in PneumaCult™-ALI-S Medium undergo extensive mucociliary differentiation to form a cuboidal epithelium that exhibits morphological and functional characteristics similar to those of the human small airway in vivo.

Together, PneumaCult™-ALI-S Medium and PneumaCult™-NGEx Medium (#100-1505) constitute a fully integrated BPE-free culture system for in vitro human small airway modeling. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.

Learn how to culture human airway epithelial cells at the ALI in our On-Demand Pulmonary Course or browse our Frequently Asked Questions (FAQs) about the ALI culture workflow using PneumaCult™.

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