Stemcell, 05790, BrainPhys™ Neuronal Medium

Promote, rather than inhibit, neuronal activity and maturity in your cultured primary or human pluripotent stem cell (hPSC)-derived neurons. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), BrainPhys™ Neuronal Medium is a serum-free basal medium that is optimized to yield a higher proportion of synaptically active neurons by mimicking the central nervous system (CNS) extracellular environment.

Use BrainPhys™ Neuronal Medium for long-term culture of hPSC- and CNS-derived neurons. To avoid shocking your cells with media changes, you can also use BrainPhys™ medium when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging.

To ensure cell health in long-term serum-free culture, BrainPhys™ Neuronal Medium must be combined with an appropriate serum-replacement supplement, such as NeuroCult™ SM1 Neuronal Supplement and/or N2 Supplement-A. For your convenience, various BrainPhys™ kits that include the required supplement(s) for primary or hPSC-derived neurons are also available.

View our additional resources to learn more about the BrainPhys™ system.