Stemcell, 05791, BrainPhys™ Without Phenol Red

Promote, rather than inhibit, neuronal activity and maturity in your cultured primary or human pluripotent stem cell (hPSC)-derived neurons in a phenol red-free environment.

Based on the formulation by Bardy and Gage (Bardy C et al. PNAS, 2015), BrainPhys™ serum-free neuronal basal medium is optimized to yield a higher proportion of synaptically active neurons by mimicking the central nervous system extracellular environment. Use BrainPhys™ Without Phenol Red to culture primary neurons, differentiate and mature hPSC-derived neurons, record microelectrode array-based neuronal activity, live-fluorescent image neurons, and transdifferentiate somatic cells to neurons without hormonal signaling.

To ensure cell survival in long-term serum-free culture, BrainPhys™ Without Phenol Red must be combined with an appropriate serum-replacement supplement, such as NeuroCult™ SM1 Neuronal Supplement (Catalog #05711) and/or N2 Supplement-A (Catalog #07152).