Stemcell, 05792, BrainPhys™ Neuronal Medium and SM1 Kit

Culture primary or human pluripotent stem cell (hPSC)-derived neurons long term in a medium optimized to promote, rather than inhibit neuronal activity and maturity.

For your convenience, BrainPhys™ Neuronal Medium and SM1 Kit includes both serum-free BrainPhys™ Neuronal Medium (basal medium) and NeuroCult™ SM1 Neuronal Supplement, a Brewer's B27-based supplement (Brewer et al. J Neurosci Res., 1993) that ensures cell health and encourages neurite outgrowth and branching in short- and long-term serum-free cultures. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), BrainPhys™ Neuronal Medium mimics the extracellular environment of the central nervous system (CNS) to yield a higher proportion of synaptically active neurons.

Use BrainPhys™ Neuronal Medium and SM1 Kit to culture primary CNS-derived neurons or embryonic stem (ES) and induced pluripotent stem (iPS) cell-derived neurons. To avoid shocking your cells with media changes, you can also use this culture system when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging.

View our additional resources to learn more about the BrainPhys™ system.