Stemcell, 05794, BrainPhys™ Primary Neuron Kit

Plate and culture primary neurons of the central nervous system (CNS) in a complete, serum-free medium optimized to promote, rather than inhibit neuronal activity and maturity.

For your convenience, BrainPhys™ Neuronal Medium and SM1 Kit includes both serum-free BrainPhys™ Neuronal Medium (basal medium) and NeuroCult™ SM1 Neuronal Supplement and NeuroCult™ Neuronal Plating Medium, to provide a complete culture system for your primary-tissue-derived neurons. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), BrainPhys™ Neuronal Medium mimics the extracellular environment of the CNS to yield a higher proportion of synaptically active neurons. NeuroCult™ SM1 Neuronal Supplement is a Brewer's B27-based supplement (Brewer et al. J Neurosci Res., 1993) that ensures cell health and encourages neurite outgrowth and branching in short- and long-term cultures.

To avoid shocking your cells with media changes, you can also use BrainPhys™ medium when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging.

View our additional resources to learn more about the BrainPhys™ system.