Stemcell, 05795, BrainPhys™ hPSC Neuron Kit

Culture, differentiate, and mature neurons derived from human embryonic stem (ES) or induced pluripotent stem (iPS) cells by using a complete medium optimized to promote, rather than inhibit neuronal activity.

For your convenience, BrainPhys™ hPSC Neuron Kit includes serum-free BrainPhys™ Neuronal Medium (basal medium), supplements, and growth factors to enable you to generate and mature different neuronal subtypes from human ES/iPS cell-derived neural progenitor cells. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), BrainPhys™ Neuronal Medium mimics the extracellular environment of the central nervous system (CNS) to yield a higher proportion of synaptically active neurons. Brewer’s B27-based (Brewer et al. J Neurosci Res., 1993) NeuroCult™ SM1 Neuronal Supplement ensures cell health and encourages neurite outgrowth and branching in short- and long-term serum-free cultures, and N2 Supplement-A supports the in vitro differentiation of ES/iPS-derived cells to neuronal subtypes. Included BDNF and GDNF growth factors support lineage-specific differentiation.

To avoid shocking your cells with media changes, you can also use BrainPhys™ medium when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging.

View our additional resources to learn more about the BrainPhys™ system.