Description
Culture primary human skeletal muscle progenitor cells and human pluripotent stem cell (hPSC)-derived myoblasts under serum-free conditions.
The MyoCult™-SF Expansion Supplement Kit (Human) includes components optimized for the derivation and expansion of myoblasts in vitro: MyoCult™-SF Expansion 10X Supplement (Human) and MyoCult™-SF Attachment Substrate. The supplement must be combined with a basal medium (DMEM with 1000 mg/L D-Glucose (Catalog #36253); sold separately) to prepare MyoCult™-SF Expansion Medium. Use of the MyoCult™-SF Attachment Substrate is optional depending on the cell source used. For your convenience, the kit components may also be purchased individually.
If you are using primary myoblasts, we recommend coating cultureware with MyoCult™-SF Attachment Substrate. For hPSC-derived myoblasts, you can use Corning® Matrigel® hESC-Qualified Matrix. Myoblasts cultured using MyoCult™-SF Expansion Medium are compatible with MyoCult™ Differentiation Kit (Human) and can be used for further differentiation into multinucleated myotubes.
For more information on protocols for derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet.
