Stemcell, 05982, MyoCult™-SF Expansion 10X Supplement (Human)

Support the serum-free culture of primary human skeletal muscle progenitor cells and human pluripotent stem cell (hPSC)-derived myoblasts using MyoCult™-SF Expansion 10X Supplement (Human).

Available either individually or as part of the MyoCult™-SF Expansion Supplement Kit (Human), the supplement has a serum-free formulation and must be combined with a basal medium (DMEM with 1000 mg/L D-Glucose (Catalog #36253); sold separately) to prepare MyoCult™-SF Expansion Medium. This medium is optimized for the derivation and expansion of human skeletal muscle progenitor cells in vitro.

If you are using primary myoblasts, we recommend coating cultureware with MyoCult™-SF Attachment Substrate. For hPSC-derived myoblasts, you can use Corning® Matrigel® hESC-Qualified Matrix. Myoblasts cultured using MyoCult™-SF Expansion Medium are compatible with MyoCult™ Differentiation Kit (Human) and can be used for further differention into multinucleated myotubes.

For more information on protocols for derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet.