Stemcell, 05985, MyoCult™ Expansion 10X Supplement (Mouse)

MyoCult™ Expansion 10X Supplement (Mouse), when combined with DMEM/F-12 to prepare MyoCult™ Expansion Medium, is for the culture of mouse skeletal muscle progenitor cells (satellite cells). MyoCult™ Expansion Medium has been optimized for the expansion of mouse skeletal muscle satellite cells isolated by fluorescence-activated cell sorting (FACS), as well as for culturing single isolated myofibers grown in suspension. Satellite cells cultured using MyoCult™ Expansion Medium can be differentiated into multinucleated myotubes.

For preparation of MyoCult™ Expansion Medium, DMEM/F-12 with 15 mM HEPES (Catalog #36254) is required in addition to MyoCult™ Expansion 10X Supplement. For expansion of satellite cells, cultureware must be coated with a matrix such as Corning Matrigel® Basement Membrane Matrix (Corning Catalog #356234).