Stemcell, 07900, DNase I Solution (1 mg/mL)

Use Deoxyribonuclease (DNase) I Solution (1 mg/mL) for routine tissue dissociation, minimizing clumping of concentrated and/or cryopreserved cell suspensions following thawing or enzymatic tissue digestion, and to eliminate DNA contaminants . The activity range of this ready-to-use solution is ≥ 2000 units per mg of protein. Consisting of a single glycosylated polypeptide chain with two disulfide bonds, DNase I is an endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.).