Stemcell, 07919, Gentle Collagenase/Hyaluronidase

Perform gentle enzymatic dissociation of mouse mammary tissue with 10X Collagenase/Hyaluronidase in Dulbecco’s Modified Eagle’s Medium (DMEM). This gentle formulation enables overnight dissociation of mouse mammary gland tissue so experiments can be completed the next day rather than late at night. Gentle Collagenase/Hyluronidase has also been used for enzymatic dissociation of mouse prostate epithelial cells (Frégeau-Proulx et al. MethodsX, 2022). This protease/polysaccharidase combination digests native collagen fibrils and hydrolizes hyaluronic acids in connective tissues for effective tissue dissociation.