Stemcell, 07923, Dispase (1 U/mL)

Use Dispase (1 U/mL) for gentle dissociation of a wide variety of tissues. This proteolytic dissociation reagent has been optimized for use in enzymatic passaging of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells. Incubation of minced tissue with pre-warmed dispase and gentle agitation will liberate cells with minimal cell damage. Pre-warmed dispase can also be used to harvest cells from tissue culture plastic.

This product contains 1 U/mL Dispase II (neutral protease from Bacillus polymyxa) dissolved in DMEM/F-12. Unlike trypsin, Dispase is not inhibited by serum. Dispase activity is inhibited by EDTA and EGTA. Dispase should be removed from cell suspensions by centrifuging the cells followed by washing the cells with buffer or culture medium.