Stemcell, 08581, STEMdiff™ SMADi Neural Induction Kit

STEMdiff™ SMADi Neural Induction Kit consists of a defined, serum-free medium and supplement for the highly efficient neural induction of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. This kit combines STEMdiff™ Neural Induction Medium (Catalog #05835) with STEMdiff™ SMADi Neural Induction Supplement, which directs differentiation by blocking TGF-β/BMP-dependent SMAD signaling, resulting in efficient neural induction of even hard-to-differentiate cell lines. Neural progenitor cells (NPCs) can be generated using STEMdiff™ SMADi Neural Induction Kit with either an embryoid body (EB) protocol or monolayer culture protocol. The resulting cultures are enriched for central nervous system (CNS)-type NPCs, which express SOX1, Nestin, and PAX6. NPCs generated using this kit can be passaged as single cells and expanded in STEMdiff™ Neural Progenitor Medium (Catalog #05833). The NPCs can also be differentiated into neurons and glia.

Learn how to generate neural progenitor cells from human pluripotent stem cells (hPSCs) in our On-Demand Neural Induction Course, and browse our Tech Tips on the Neural Induction of hPSCs using the Embryoid Body Method or Monolayer Method.