Stemcell, 09707, HemaTox™ Megakaryocyte Kit

Screen large numbers of novel drug compounds for their effect on the proliferation and megakaryocyte-specific differentiation of human hematopoietic stem and progenitor cells (HSPCs).

Compared to using the colony forming unit (CFU) assay, the liquid culture-based HemaTox™ assay is more flexible: you can add test compounds at various points throughout the culture and measure their effects on more primitive or more mature progenitor cells. The HemaTox™ Megakaryocyte Kit promotes the proliferation of human CD34+ HSPCs and their differentiation into CD41-expressing megakaryocytes.

Users can choose the readout method to quantify the response and estimate the IC50 and IC90 of each compound tested. Test up to 160 conditions—in triplicate—per kit in 5 x 96-well plates. This kit may be used on its own or in combination with HemaTox™ Erythroid Kit (Catalog #09701) or HemaTox™ Myeloid Kit (Catalog #09704)to assess lineage-specific drug toxicity in parallel.