Description
Easily isolate highly purified and magnetic particle-free human CD271+ cells from human pluripotent stem cell (PSC)-derived neural crest cell culture samples, using immunomagnetic positive selection, with the EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CD271 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated human PSC-derived neural crest cells using a release agent. The final isolated fraction contains highly purified CD271+ cells that are immediately ready for downstream applications such as flow cytometry or cell culture. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
