Stemcell, 100-0170, STEMdiff™ NK Cell Kit

Differentiate human pluripotent stem cells (hPSCs) to NK cells with the feeder-free and serum-free STEMdiff™ NK Cell Kit.

The STEMdiff™ NK Cell Kit protocol first uses animal component-free STEMdiff™ Hematopoietic - EB reagents for the generation of embryoid bodies (EBs) from hPSCs which are then further differentiated into CD34+ cells. The kit includes all the EB reagents necessary for this step:

• STEMdiff™ Hematopoietic - EB Basal Medium

• STEMdiff™ Hematopoietic - EB Supplement A

• STEMdiff™ Hematopoietic - EB Supplement B

The CD34+ cells are then further directed to differentiate to CD56+ NK cells using StemSpan™ reagents:

• StemSpan™ SFEM II

• StemSpan™ Lymphoid Progenitor Expansion Supplement (10X)

• StemSpan™ Lymphoid Differentiation Coating Material (100X)

• StemSpan™ NK Cell Differentiation Supplement (100X)

For your convenience, the STEMdiff™ Hematopoietic - EB and StemSpan™ reagents included with the kit are also available individually.