Stemcell, 100-0247, Thiazovivin

Thiazovivin is a selective inhibitor of Rho-associated coiled-coil containing protein kinase (ROCK), a serine/threonine kinase that plays a role in cell polarity, contraction, and actin cytoskeleton reorganization (Xu et al.). Thiazovivin is effective at 5-fold-lower concentrations than another common ROCK inhibitor Y-27632 (Catalog #72302; Xu et al.).

MAINTENANCE AND SELF-RENEWAL

· Promotes survival of human embryonic stem (ES) cells during dissociation by stabilizing E-cadherin and improves cell attachment (Xu et al.).

· Promotes survival of single human induced pluripotent stem (iPS) cells during transfection for TALEN-mediated genome editing (Sun and Zhao).

REPROGRAMMING

· Increases the efficiency of reprogramming human somatic cells to iPS cells, in combination with PD0325091 and SB431542 (Lin et al.).

· Increases the efficiency of reprogramming human cord blood mononuclear cells to iPS cells (Hu et al.).