Stemcell, 100-0350, EasySep™ Mouse TIL (CD45) Positive Selection Kit

Easily isolate highly purified mouse CD45+ tumor-infiltrating leukocytes (TILs) by immunomagnetic positive selection using the EasySep™ Mouse TIL (CD45) Positive Selection Kit. This kit has been optimized for use on single-cell suspensions of solid mouse tumors, including tumors induced by implantation of 4T1, B16-F10, and CT26.WT cell lines into syngeneic mice. Due to the heterogeneity of mouse tumors, this kit may require optimization. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD45 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD45+ TILs are ready for downstream applications such as flow cytometry, culture, and cell-based experiments.

Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.