Stemcell, 100-0352, Conditional Reprogramming (CR) Medium

Conditional Reprogramming (CR) Medium is optimized to use with 3T3-J2 Irradiated Feeder Cells (Catalog #100-0353) to expand epithelial cells without genetic modification. Mature epithelial cells have been shown to restore tissue-specific progenitor phenotype that enables proliferation in the presence of CR Medium (Liu X et al. 2012; Liu X et al. 2017). This conditional proliferative capability is deactivated once the medium is removed from the cells (Liu X et al. 2012; Liu X et al. 2017). CR Medium is suitable to propagate primary epithelial cells from healthy or tumor tissues (airway, retina, prostate, breast, intestine, pancreas, liver biliary duct, etc.; Liu X et al. 2012; Liu X et al. 2017; Suprynowicz FA et al.). This product is free of antibiotics and intended for Research Use Only. CR Medium must be supplemented with cholera toxin prior to use. To learn more, read our tech tip on the mechanisms and applications of CR technology.