Stemcell, 100-0528, STEMdiff™ Branching Lung Organoid Maturation Kit

Mature and maintain human branching lung organoids in long-term (>28 days) culture using STEMdiff™ Branching Lung Organoid Maturation Kit.

Serum-free STEMdiff™ Branching Lung Organoid Maturation Kit may be used to reproducibly mature human embryonic stem (ES) and induced pluripotent stem (iPS) cell-derived lung bud organoids generated using STEMdiff™ Branching Lung Organoid Kit. Resulting organoids will develop proximal and distal-like branching airway epithelial structures expressing EPCAM, NKX2.1, SOX2, SOX9, MUC1, and P63. When maintained beyond 28 days in a Matrigel® sandwich, there is an increase in expression levels of markers for more mature lung cells, such as SFTPC, SFTPB, and ABCA3.

For detailed information on protocols for initiating and maturing branching lung organoids using the STEMdiff™ Branching Lung Organoid media system, please refer to the Product Information Sheet (PIS).