Stemcell, 100-0536, Ac-DEVD-CHO

Ac-DEVD-CHO is a synthetic peptide aldehyde with the PARP cleavage site DEVD, which is recognized by caspases (Nicholson et al.). Ac-DEVD-CHO blocks PARP cleavage activity and inhibits caspase-3 (Ki = 0.23 nM) and caspase-7 (Ki = 1.6 nM) in a reversible manner (Garcia-Calvo et al.; Talanian et al.). This inhibition is via the aldehyde group’s interaction with the active site cysteine of these caspases (Thornberry et al.). Caspase inhibitors are used to investigate caspase activation and apoptosis. This product is supplied as the trifluoroacetate salt of the molecule.

CANCER RESEARCH

· Blocks camptothecin-induced apoptosis in bone tumor cells (Nicholson et al.).