Stemcell, 100-0620, PneumaCult™ Apical-Out Airway Organoid Medium

Generate apical-out airway organoids in 15 days from human bronchial epithelial cells (HBECs) or human airway epithelial cells (HAECs) with a simple and scalable, Matrigel®-free protocol. These organoids provide access to the apical side of the airway epithelium and can be used to perform your infectious disease modeling or high-throughput drug screening in an in vitro, three-dimensional (3D) model system.

With PneumaCult™ Apical-Out Airway Organoid Medium, you can grow polarized airway organoids with outward-facing, differentiated ciliated cells in AggreWell™400 24-well plates (Catalog #34415)—without the use of cell culture inserts. To maximize experimental reproducibility, this medium is formulated without serum or BPE. For optimal results, begin your workflow by expanding HAECs in PneumaCult™-NGEx Medium (Catalog #100-1505) prior to differentiation.

Find more about the applications of lung organoids in our learning center.