Description
Easily isolate or deplete human CD11b+ cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples, using immunomagnetic positive selection, with the EasySep™ Human CD11b Positive Selection and Depletion Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, cells of interest are labeled with antibody complexes recognizing CD11b and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unlabeled cells. The CD11b+ cells remain in the tube. Following magnetic cell isolation in as little as 16 minutes, desired cells from either fraction are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. This kit targets human CD11b, a subunit of the integrin macrophage-1 antigen (Mac-1) receptor commonly used as a myeloid cell marker. CD11b is expressed on immune cells such as monocytes, macrophages, neutrophils, and NK cell subsets, and is known to play a role in regulating cell adhesion, migration, and phagocytosis (Khan SQ et al. Front Med, 2018).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
