Description
Grow, expand, and differentiate organoids from human alveolar epithelial cells. Unlike traditionally used animal models, alveolar organoids mimic the regional specificity of the in vivo alveoli, making them ideally suited for alveolar biology research, infectious disease studies, and drug screening. With the convenience of PneumaCult™ Alveolar Organoid Media, you can efficiently passage and expand human alveolar epithelial type II (ATII) cells long term as organoids, then further differentiate these ATII organoids to alveolar epithelial type I (ATI) cells for downstream applications.
Use PneumaCult™ Alveolar Organoid Expansion (AvOE) Medium to achieve a 10,000X cumulative-fold expansion of ATII cells as organoids within 10 passages; this high yield reduces the need to regularly source donor tissue. You can flexibly pause your cultures at any passage by cryopreserving organoids cultured in PneumaCult™ AvOE Medium as single cells. The medium’s three-component, serum-free format maximizes experimental reproducibility, and the alveolar organoids maintain properties indicative of the ATII cell phenotype, including the ability for self-renewal, expected marker expression (e.g. HT2-280 and SP-C), and lineage potential for differentiation to ATI cells. PneumaCult™ AvOE Medium can also be used to generate alveolar monolayers to facilitate air-liquid interface (ALI) culture as outlined in this protocol.
Use PneumaCult™ Alveolar Organoid Differentiation (AvOD) Medium to differentiate ATII cells expanded as organoids in PneumaCult™ AvOE Medium. The two-component PneumaCult™ AvOD Medium enables in-dome differentiation of ATII organoid cultures to ATI cells in as few as 10 days, with over 85% purity. Differentiated cells show decreased ATII marker expression and strong up-regulation of ATI cell markers (RAGE/AGER, HT1-56, and GPRC5a).
PneumaCult™ AvOE and AvOD Media are compatible with primary isolated fresh or cryopreserved ATII cells, as well as high-quality commercially available alveolar epithelial cell sources.
Learn more about lung organoid cultures in our learning center.
