Stemcell, 100-0856, Human Recombinant TGF-beta 1 (Cell Culture Ready)

Conveniently and consistently add transforming growth factor beta 1 (TGF-ꞵ1) to your cultures with this cell culture-ready formulation. This human recombinant TGF-ꞵ1 is precisely reconstituted to 0.1 mg/ml in 100 mM acetic acid and requires no additional preparation, improving reproducibility. Endotoxin levels are verified, using the LAL method to ensure consistency for use across multiple applications. A member of the TGF-β superfamily, TGF-β1 regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins.

TGF-β1 has been used for:

hPSC CULTURE MAINTENANCE

● Together with basic fibroblast growth factor (bFGF), supports the culture of undifferentiated human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells (Amit et al. Biol Reprod, 2004).

EXOGENOUS CELL SIGNALING

● Regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (Massagué Nat Rev Mol Cell Biol, 2000; McDowell et al. Curr Biol, 1997).

IMMUNE CELL HOMEOSTASIS

● Modulate B cell responses and mediate immunosuppressive effects on T cells and neutrophils (Letterio & Roberts Annu Rev Immunol, 1998).