Stemcell, 100-0871, STEMdiff™ Motor Neuron Differentiation Kit

With STEMdiff™ Motor Neuron Differentiation Kit, based on the established protocol by Maury et al. (Nature Biotechnology, 2015), you can produce pure populations of spinal motor neurons with an average of >80% Class III β-tubulin positive, >50% motor neuron marker ISL1 positive, >60% motor neuron marker HB9 positive. These motor neurons also exhibit high expression of HOXA5. Use the resulting motor neuron cultures in a variety of applications, such as modeling human neurological disease or toxicity testing. These highly versatile cultures are suitable for use in neuromuscular co-culture with myotubes generated using MyoCult™ Differentiation Kit (Catalog #05965), in co-culture with microglia generated using STEMdiff™ Microglia Differentiation Kit (Catalog #100-0019), or with AggreWell™400 (Catalog #34460) for higher yield production.

For further maturation of motor neurons generated with this kit, pair with STEMdiff™ Motor Neuron Maturation Kit to produce >40% ChAT cells in an additional 14 days.