Description
Easily and efficiently isolate highly purified human natural killer (NK) cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human NK Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, monocytes, dendritic cells, erythroid cells. The magnetically labeled cells are then separated from the untouched desired NK cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 8 minutes, the desired NK cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This product replaces EasySep™ Human NK Cell Enrichment Kit (Catalog #19055) for even faster NK cell negative isolation.
For NK cell isolation, including emerging NK cells, we recommend the EasySep™ Human Pan-NK Cell Isolation Kit (Catalog #100-1580), which isolates NK cells containing CD36+ and HLA-DR+ emerging subsets in as little as 8 minutes.
For large-scale isolation of human NK cells from leukapheresis samples, see the large-format (1x10^10 cells) kit (Catalog #100-0960).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood NK Cells, Fresh isolated with EasySep™ Human NK Cell Isolation Kit. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
