Stemcell, 100-0992, TMRE (Perchlorate)

Detect changes in mitochondrial membrane potential with TMRE (Perchlorate) dye. TMRE uses a quick and simple staining protocol that is well suited for high-throughput screening and is compatible with flow cytometry.

Features:

• Excitation wavelength: 552 nm

• Emission wavelength: 574 nm

• Retention dependent on mitochondrial inner membrane potential

• Verified for use with flow cytometry

TMRE is a cell-permeant, cationic compound that emits a fluorescent red-orange signal. It readily accumulates in the mitochondria with high membrane potential, whereas depolarized mitochondria fail to retain the dye.

TMRE has a sufficient retention time that is optimal for cell sorting for further purification and characterization (Barteneva et al. J Histochem Cytochem, 2014). TMRE does not inhibit the mitochondrial function, and can be used in the identification of apoptotic cells (Barteneva et al., 2014).