Stemcell, 100-1024, CellPore™ FITC-Dextran

Optimize mechanoporation-based delivery with the CellPore™ Transfection System by using CellPore™ FITC-Dextran, a green fluorescent tracer designed to assess transfection efficiency. CellPore™ FITC-Dextran comes in a ready-to-use format, making it an easy-to-use universal positive control cargo for all your applications.

This hydrophilic polysaccharide, consisting of dextran labeled with fluorescein isothiocyanate (FITC), is characterized by minimal toxicity and low immunogenicity, enabling reliable downstream analysis with minimal impact on cell health.

During mechanoporation, transient membrane permeabilization enables rapid FITC-dextran entry, resulting in markedly stronger intracellular fluorescence compared to natural uptake by endocytosis. Use CellPore™ FITC-Dextran as a positive control to determine the optimal CellPore™ delivery pressure for your cell type and application. Following your initial optimization run, select the pressure that maximizes uptake while preserving viability for subsequent experiments. CellPore™ FITC-Dextran can also be co-delivered with other functional cargo to track transfection efficiency in parallel with functional assays. Delivery performance can be analyzed by flow cytometry, and the marker is compatible with multi-color staining panels.

For best results, use in conjunction with the CellPore™ Transfection System, CellPore™ Delivery Cartridges, and CellPore™ Delivery Media.

For more information about CellPore™, including the intracellular delivery technology used in the CellPore™ Transfection System, visit the CellPore™ overview page.