Stemcell, 100-1041, A 83-01

A 83-01 is a potent inhibitor of activin receptor-like kinase (ALK) including ALK5 (type I transforming growth factor-β receptor), ALK4 (type IB activin receptor), and ALK7 (type I NODAL receptor) with IC₅₀ = 12, 45, and 7.5 nM, respectively (Tojo et al.).

MAINTENANCE AND SELF-RENEWAL

· Facilitates the conversion of epiblast stem cells to the naїve pluripotent state (Zhou et al.; Li et al.).

REPROGRAMMING

· In combination with PD0325901, enables OCT4-mediated reprogramming (Zhu et al.).

DIFFERENTIATION

· Inhibits mesoderm and endoderm specification (Zhang et al.).

· Disrupts epithelial to mesenchymal transition through inhibition of the SMAD signaling pathway (Tojo et al.).