Stemcell, 100-1044, Y-27632 (Dihydrochloride)

Y-27632 is a cell-permeable, highly potent and selective inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK). Y-27632 inhibits both ROCK1 (Ki = 220 nM) and ROCK2 (Ki = 300 nM) by competing with ATP for binding to the catalytic site (Davies et al.; Ishizaki et al.).

MAINTENANCE AND SELF-RENEWAL

· Enhances survival of human embryonic stem (ES) cells when they are dissociated to single cells by preventing dissociation-induced apoptosis (anoikis), thus increasing their cloning efficiency (Watanabe et al.).

· Improves embryoid body formation using forced-aggregation protocols (Ungrin et al.).

· Increases the survival of cryopreserved single human ES cells after thawing (Li et al.).

· Blocks apoptosis of mouse ES-derived neural precursors after dissociation and transplantation (Koyanagi et al.).

REPROGRAMMING

· Direct lineage reprogramming of fibroblasts to mature neurons, in combination with CHIR99021, RepSox, Forskolin, SP600125, Gö6983 and Valproic Acid (Hu et al.).

DIFFERENTIATION

· Improves survival of human ES cell monolayers at the initiation of differentiation protocols (Rezania et al.)