Stemcell, 100-1046, DAPT

DAPT is an inhibitor of the γ-secretase complex. Notch is a key target of γ-secretase, therefore DAPT indirectly inhibits the Notch pathway. Other targets of γ-secretase that would be influenced by DAPT include amyloid precursor protein, E-cadherin, and ErbB4. (Dovey et al.).

MAINTENANCE AND SELF-RENEWAL

· Reduces colony-forming efficiency of mouse neural stem cells (Androutsellis-Theotokis et al.).

· Enhances radiation-induced cell death of glioma stem cells (Wang et al.).

DIFFERENTIATION

· Promotes differentiation of nociceptors from human pluripotent stem cells, in combination with several other small molecules (Chambers et al.).

· Promotes differentiation of neurons from human and mouse embryonic stem (ES) cells (Crawford and Roelink, Elkabetz et al.).

· Promotes differentiation of retinal pigment epithelium from mouse ES cells (Osakada et al.).

· Promotes differentiation of pancreatic cells from human pluripotent stem cells (D'Amour et al.).

CANCER RESEARCH

· Reduces mammosphere-forming efficiency of breast cancer cell lines and ductal carcinoma in situ cells (Farnie et al., Harrison et al.).