Description
Easily isolate highly purified cells expressing the CD138 surface antigen from fresh human whole bone marrow samples, using immunomagnetic positive selection, with the EasySep™ Human Bone Marrow CD138 Positive Selection Kit (IVD). This kit is an in vitro diagnostic (IVD) medical device for hematopoietic cell enrichment available in Australia, Canada, the European Union (EU), Switzerland, Turkey, the United Kingdom (UK), and the United States, and is indicated for use with validated diagnostic assays as part of the pre-analytical workflow. The intended use of this kit is to enrich bone marrow samples, from patients who may or are known to have multiple myeloma, for CD138+ cells using immunomagnetic positive selection. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD138 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD138+ cells are ready for use in validated downstream diagnostic assays. The end-user is responsible for validation of this kit for use with the assay. For in vitro diagnostic use by laboratory professionals.
The National Comprehensive Cancer Network® (NCCN Guidelines Version 2.2020 Multiple Myeloma) recommends using enriched plasma cells from bone marrow aspirations when performing fluorescence in situ hybridization (FISH) analysis for multiple myeloma testing. Enriching bone marrow samples for CD138+ cells has been reported to increase the rate and frequency of chromosomal abnormalities detected by FISH (e.g. Shin SY et al. Int J Hematol, 2012; Shetty S et al. Int J Hematol, 2012), which may help define effective treatment strategies against multiple myeloma.
Learn more about isolating CD138+ plasma cells to enhance FISH sensitivity for multiple myeloma in this on-demand training course.
Note: Ref #100-0748 is the Regulatory Product ID and should not be used for ordering.
Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
