Stemcell, 100-1215, eTeSR™

Improve the genetic stability of human pluripotent stem cell (hPSC) cultures when performing routine single-cell passaging by using eTeSR™, an enhanced feeder-free culture medium. eTeSR™ builds upon previous formulations of TeSR™ (Ludwig et al. Nat Biotechnol, 2006; Ludwig et al. Nat Methods, 2006), the most widely published feeder-free cell culture media for hPSCs.

Specifically developed for single-cell applications, which typically involve shorter passaging schedules at high density, eTeSR™ can be used for routine maintenance, gene editing, or cloning workflows. Use eTeSR™ to produce high-quality hPSCs with improved genetic stability for these applications; stabilization of the components (e.g. FGF2), optimized metabolites, and improved buffering capacity address the increased metabolic demand and cell stress associated with single-cell passaging. Expect equivalent high performance when using eTeSR™ with either daily or restricted feeding schedules.

eTeSR™ is compatible with a variety of cell culture matrices, including Corning® Matrigel® hESC-Qualified Matrix (Corning Catalog #354277) and CellAdhere™ Laminin-521 (Catalog #200-0117).

Each lot of eTeSR™ 10X Supplement is quality-tested in a culture assay using hPSCs.