Stemcell, 100-1340, Anti-Human PAX6 (AN) Antibody, Polyclonal

This rabbit polyclonal antibody reacts with human and mouse paired box protein Pax6, a ~46 kDa protein encoded by PAX6 gene belonging to the PAX family of transcription factors. Use this polyclonal antibody in applications requiring detection of PAX-6, such as ELISA, immunohistochemistry (IHC), western blotting (WB), immunocytochemistry (ICC), and immunofluorescence (IF). This antibody clone has been verified for labeling PAX6-positive hPSC-derived neural stem and progenitor cells generated with STEMdiff™ SMADi Neural Induction Kit (Catalog #08581) and STEMdiff™ Neural Progenitor Medium (Catalog #05833).

Expressed in the brain, eyes, pancreas, and spinal cord, Pax-6 potentiates their embryonic development, as well as the maintenance of glucose homeostasis and function of the endocrine pancreas. PAX6 has at least 3 isoforms generated via alternative mRNA splicing, i.e., PAX6, PAX6(5a), and PAX6(ΔPD). PAX6 contains two DNA-binding domains, the paired domain (PD) and a paired-type homeodomain (HD) connected by a linker region. Downstream of the HD, a P/S/T-rich domain at the C-terminal acts as an activator of gene expression. The PAX6(5a) splice variant contains a 14 amino acid insert into the PD, altering the specificity of its DNA binding activity, while the PAX6(ΔPD) isoforms lack a PD, resulting in a truncated protein. In humans, mutations of the PAX6 gene resulted in aniridia and related developmental eye diseases, while Pax-6 deficiency in adult mice resulted in progressive hyperglycemia.