Stemcell, 100-1341, Anti-Human TBR1 (IDDAS) Antibody, Polyclonal

This rabbit polyclonal antibody reacts with human, mouse and rat T-box brain 1 (TBR1), a transcription factor highly expressed and involved in human development; mutations of the T-box gene have been linked to developmental disorders as well as the manifestation of certain cancers. Use this polyclonal antibody in applications requiring detection of TBR1, such as ELISA, western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF). This antibody clone has been verified for labeling TBR1-positive cortical neurons in hPSC-derived cerebral organoids generated with STEMdiff™ Cerebral Organoid Kit (Catalog #08570) and STEMdiff™ Cerebral Organoid Maturation Kit (Catalog #08571).

The T-box transcription factor family consists of five subfamilies, with the TBR1 family of proteins responsible for brain development, primarily through the function of T-box proteins that act as transcriptional activators and inhibitors. In particular, the function of TBR1 in brain development is primarily facilitated by the differentiation of neural stem cells (NSCs) in both developing and adult brains. Structurally, T-box transcription factors contain a conserved DNA-binding motif named T-box domain, spanning 180-200 amino acid residues to bind DNA. Studies involving TBR1-deficient mice found defects in the frontal cortex and layer 6 differentiation.