Stemcell, 100-1367, STEMdiff™-ACF RPE Differentiation Kit

Rapidly and robustly generate hPSC-derived immature retinal pigment epithelium (RPE) in 14 days with STEMdiff™-ACF RPE Differentiation Kit. This animal component- and serum-free kit generates immature RPE (> 50% PMEL17+) from hPSCs in clump culture maintained in mTeSR™1 or mTeSR™ Plus.

Immature RPE generated using STEMdiff™-ACF RPE Differentiation Kit can be cryopreserved as an intermediate cell bank or matured further into functional RPE using STEMdiff™-XF RPE Maturation Medium. By using these products together, a highly pure population of RPE (Figure 2) can be generated from hPSCs in as few as 49 days without the need for manual selection or cell enrichment. Increase the yields and survivability of your RPE cell differentiation and maturation by adding the STEMdiff™-ACF RPE Plating Supplement to this workflow.

Cells derived using these products can be used for modeling human retinal development and disease, drug screening, cell and gene therapy validation, and advanced tissue model development.

Please contact us if you intend to use this product for commercial or clinical applications.