Description
Achieve robust airway epithelial cell cultures with PneumaCult™-NGEx Medium, our third-generation medium designed to be the new gold standard for the expansion of human nasal, bronchial, and small airway epithelial cells. Building upon previous versions of PneumaCult™ expansion media, PneumaCult™-NGEx is a convenient, two-component, serum- and BPE-free medium offering 250x more cells in three weeks—outperforming do-it-yourself (DIY) formulations and other commercially available expansion media.
PneumaCult™-NGEx can support your cultures through extended passaging to beyond Passage 15, while maintaining the in-vivo cobblestone morphology. Importantly, airway basal cells expanded long-term in PneumaCult™-NGEx show better-conserved differentiation potential and function compared to those cultured in other commercially available expansion media. This is demonstrated by stable expression of key genes associated with basal cell maintenance and differentiation over long-term culture, such as KRT5 and p63. PneumaCult™-NGEx therefore ensures a reliable source of high-quality airway epithelial cells throughout your experiments without having to initiate new cultures over time.
Airway epithelial cells expanded in PneumaCult™-NGEx are compatible with our other downstream differentiation workflows, enabling you to model the human lung at the air-liquid interface (ALI) or as organoids on a unified platform. These physiologically relevant models are valuable tools for basic respiratory research, toxicity studies, and drug development, which all begin on a foundation of robust airway epithelial cells expanded in culture.
To learn more about culturing human airway epithelial cells at the ALI or as organoids, register for our free, On-Demand Pulmonary Course, or browse our frequently asked questions on ALI and Airway Organoid Cultures.
