Stemcell, 100-1524, STEMdiff™ Spinal Cord Organoid Differentiation Kit

Robustly generate three-dimensional, patterned spinal cord organoid cultures from human pluripotent stem cells without matrix embedding by using STEMdiff™ Spinal Cord Organoid Differentiation Kit. When paired with AggreWell™800, this serum-free cell culture media can prevent organoid fusion and support the generation of up to 300 organoids per kit for higher-powered statistical replicates and more detailed longitudinal study.

Adapted from protocols by Dr. Sergiu Paşca (Andersen J et al. Cell, 2020; Yoon SJ et al. Nature Methods, 2018), the spinal cord organoids generated with this kit are 3D in vitro models with a cellular composition and structural organization representative of the developing human cervical spinal cord. They are consistently sized with reproducible morphology between various cell lines and provide a reliable model for the study of motor neurons within a functional 3D system.

Organoids generated with this kit can also be co-cultured as AssemBloids™ to study brain region interactions (Andersen J et al. Cell, 2020). For extended periods of organoid culture (> 50 days), organoids can be maintained with STEMdiff™ Neural Organoid Maintenance Kit to support long-term culture survival for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays.