Stemcell, 100-1542, ATP Assay, Bioluminescence

ATP Assay, Bioluminescence is a one-step, 20-minute assay that measures ATP to monitor the number of viable mammalian cells in cell proliferation, cytotoxicity, and other cell-based assays. This assay uses firefly luciferase, a 61 kDa monomeric enzyme, responsible for catalyzing the oxidation of firefly luciferin. This reaction produces a stable and long-lasting (> 2 hours) light emission of 560 nm, which directly corresponds to the concentration of ATP present in the cells. This assay can reliably detect as few as 50 cells per well and is free of unpleasant odors, as the firefly luciferase does not require enzymatic stabilization with dithiothreitol (DTT). ATP Assay, Bioluminescence is supplied as a single, ready-to-use reagent (no washing of cells required) and can be utilized in 96- or 384-well microplates, making it an ideal solution for high-throughput screening experiments. Quantification can be carried out using a luminescent capable microplate reader (luminometer).