Stemcell, 100-1570, EasySep™ Release Mouse CD4+CD304+ Regulatory T Cell Isolation Kit

Isolate CD4+CD304+ regulatory T cells (Tregs) from single-cell suspensions of mouse splenocytes or lymph nodes, in less than 45 minutes, using column-free immunomagnetic positive selection. First, CD304+ cells are isolated using EasySep™ Release technology. Then, bound magnetic particles are removed from the EasySep™-isolated CD304+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD304+ cells that express high levels of FOXP3 and CD25 Treg markers and are immediately ready for downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction. An optional protocol allows for the isolation of CD4+CD304- responder T cells in parallel for use in functional studies.

This kit is a first-of-its-kind mouse Treg isolation kit that targets the CD304 surface marker, which, unlike the CD25 Treg marker commonly used for Treg isolation, does not interfere with IL-2 signaling. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.