Stemcell, 100-1598, Anti-Mouse CD45.2 Antibody, Clone 104

This monoclonal antibody (clone 104) reacts with mouse alloantigen cluster of differentiation 45.2 (CD45.2), also known as Ly5.2 and leukocyte common antigen, a 180 -240 kD type I transmembrane glycoprotein. CD45.2 is an allelic form of CD45 expressed by all hematopoietic cells except mature erythrocytes and platelets in mouse strains 129, A, AKR, BALB/c, C3H/He, C57BL/6, C58, CBA, DBA/1, and DBA/2. Use this recombinant monoclonal antibody in applications requiring detection of CD45.2, such as flow cytometry (FC). This antibody clone has been verified for purity assessment of cells isolated with EasySep™ Mouse CD45 Positive Selection Kit (Catalog #18945) or EasySep™ Mouse TIL (CD45) Positive Selection Kit (Catalog #100-0350).

Clone 104 does not react with leukocytes that express the CD45.1 alloantigen. CD45 is a member of the protein tyrosine phosphatase (PTP) family and contains two catalytic domains in the intracellular (COOH) domain, which regulate cellular signaling. The extracellular domains are highly variable due to alternative splicing among exons 4, 5, and 6 as well as differential glycosylation. These CD45 isoforms correlate to specific cell types as well as specific activation and maturation states of immune cells. This monoclonal antibody has been used extensively in adoptive cell transfer and bone marrow transplantation studies in mice, allowing for the differentiation of CD45.2+ and CD45.1+ cells.