Stemcell, 100-1624, Anti-Mouse CD80 (B7-1) Antibody, Clone 16-10A1

This monoclonal antibody reacts with mouse cluster of differentiation 80 (CD80), a 55 kDa type I transmembrane protein ligand that is part of the immunoglobulin superfamily. CD80 is expressed by macrophages, dendritic cells, and activated B cells. CD80 is closely related to and works in tandem with CD86 (B7-2) to prime T cells. It has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4), and can deliver an inhibitory signal to T cells. Use this recombinant monoclonal antibody in applications requiring detection of CD80, such as flow cytometry (FC).

CD80 is significantly involved in immune cell activation in response to pathogens and acts as a cellular attachment receptor for adenovirus subgroup B. It is thought that CD80 interacts with a ligand on natural killer cells thus activating the natural killer cell-mediated cell death of the CD80 carrier. This phenomenon has potential as a possible cancer immunotherapy through the induction of CD80 expression on tumor cells. This antibody can be used as a marker to assess classically activated M1 murine macrophages.