Stemcell, 100-1627, Anti-Mouse CD86 (B7-2) Antibody, Clone GL-1

This monoclonal (clone GL-1) antibody reacts with mouse cluster of differentiation 86 (CD86; also known as B7-2), an ~80 kDa immunoglobulin superfamily member and a type I transmembrane glycoprotein. Both CD80 and CD86 are ligands of the T cell surface proteins CD28 and CD152 (CTLA-4); however, CD86 is expressed earlier in the immune response compared to CD80. CD86 is also involved in immunoglobulin class-switching and triggering of natural killer cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 with a 20- to 100-fold higher affinity than CD28 and deliver an inhibitory signal to T cells as well as down regulate the immune response. CD86 has been used as a phenotypic marker for differentiating cells. This antibody can be used as a marker to assess classically activated M1 murine macrophages. Use this recombinant monoclonal antibody in applications requiring detection of CD86, such as flow cytometry (FC).