Stemcell, 100-1666, STEMdiff™ Astrocyte Serum-Free Maturation Kit

Rapidly and efficiently mature cortical-type astrocytes from astrocytic precursors using the STEMdiff™ Astrocyte Serum-Free Maturation Kit. Used in conjunction with the STEMdiff™ Astrocyte Differentiation Kit, astrocytic precursor cells are generated from human pluripotent stem cells (hPSCs) before being matured into astrocytes using this kit. Use this completely serum-free system to obtain a highly pure population of astrocytes (an average of > 70% S100B-positive and > 60% GFAP-positive astrocytes; < 15% doublecortin-positive neurons) from hPSCs in as few as 7 weeks, and choose to maintain them long term in culture. This kit displays greater consistency among different cell lines for marker expression and glutamate uptake. Cells derived using these products are versatile tools for modeling human neurological development and disease, drug screening, and toxicity testing.